Insertion junction: CLIP2.076371_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.076371
Chromosome:chromosome 2
Location:4739303
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g108400 DAD1 Putative defender against death (DAD) protein; (1 of 1) K12668 - oligosaccharyltransferase complex subunit epsilon (OST2, DAD1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGTTGGTCGGGTGTCAGGCACAACCGATGAGCTGTGTACTCTGAAGCT
Internal bar code:CTAATACTGAATAAAATCACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:4322
LEAP-Seq percent confirming:98.6301
LEAP-Seq n confirming:72
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:73

Suggested primers for confirmation by PCR

Suggested primer 1:AGTTCACTCAGCTGCCGTAC
Suggested primer 2:AGCCAACAAGGAGTTCTCGG