| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.076374 |
| Chromosome: | chromosome 12 |
| Location: | 788325 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492250 | TRZ3 | RNase Z-like protein; (1 of 3) K00784 - ribonuclease Z (rnz) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCCTACAGCTGCACCGCCAACACTGAACCGCCACAAACTGGCGCCTA |
| Internal bar code: | ATGTGTAGTGTGTTCTAAGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2514 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGTATCTGCCTGCTGTAG |
| Suggested primer 2: | ACACCAACCTCTGCACGTAG |