| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076378 |
| Chromosome: | chromosome 1 |
| Location: | 2438263 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g014100 | (1 of 1) PTHR14614//PTHR14614:SF29 - HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN // PROTEIN FAM86A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCATCATCCGCCGGACCTGGAAGCCCGCCGGGGGCCAAGTTGCATATG |
| Internal bar code: | TGCCCTCGCCTAGAGTAGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1120 |
| LEAP-Seq percent confirming: | 10.9091 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGGCTACGGTTGTTGAGG |
| Suggested primer 2: | TCGGTACTCAAGGGTCGACT |