Insertion junction: CLIP2.076388_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.076388
Chromosome:chromosome 12
Location:4897614
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre12.g524000 SUC1,GTR7 (1 of 1) 2.4.1.14 - Sucrose-phosphate synthase / UDP-glucose-fructose-phosphate glucosyltransferase; Sucrose-phosphate synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACGCCTCTCTTGACACGCCCGCATCACTGCCCGGGCTCCTTTCCCTCG
Internal bar code:GTCTCACTTCAGGAAATTCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:707
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGGGAGGAGGCTTGAGAG
Suggested primer 2:ACGGAAGGTCTTGCAGCTAC