Insertion junction: CLIP2.076409_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.076409
Chromosome:chromosome 6
Location:5049474
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre06.g282100 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACAAACACACAGGTGTACACTGTCGACCCTTCCTTCCACAAATCCACA
Internal bar code:TCCAGGCAATCGTAAATTGCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:171
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CCACTTTCACACACTGCTGC
Suggested primer 2:GTACCGCTGCTCCATCCTAC