Insertion junction: CLIP2.076413_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.076413
Chromosome:chromosome 16
Location:1177921
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g650500 (1 of 1) K06195 - ApaG protein (apaG) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACTAATCCCCTGCATTTCCTGCTTGAGGGTTTGCCACAGCAAGAATCGC
Internal bar code:AATCGGTCTGGACGAGTCATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:329
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GTCAGGATCTGGTGTGTGGG
Suggested primer 2:CACTCACCGCTTGATCACCT