Insertion junction: CLIP2.076429_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.076429
Chromosome:chromosome 5
Location:2687807
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre36.g759597 (1 of 2) IPR001841//IPR013083//IPR024766 - Zinc finger, RING-type // Zinc finger, RING/FYVE/PHD-type // Zinc finger, RING-H2-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGACAAGATACCCCCCCATTCACACGCATCAGCGCCAAAGACCCGACGGG
Internal bar code:ACACCACTGTTATTCACAAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:402
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCTGTGTGTGAGGCAGAT
Suggested primer 2:TGCTGACTGTGTGTGTGGTT