| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.076446 |
| Chromosome: | chromosome 4 |
| Location: | 1949224 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218750 | THB4 | Truncated hemoglobin; (1 of 1) IPR012292 - Globin/Protoglobin | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCATGAATCTAAATGCGCTTAGTCAAGCGTGCCCCCAACCCTTAGCAG |
| Internal bar code: | TAGATCACAGTGTAAGGCATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2866 |
| LEAP-Seq percent confirming: | 78.3784 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGAAGATCGAGAAGGCGG |
| Suggested primer 2: | TGCCGAACAACGTCTCATCA |