| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.076459 |
| Chromosome: | chromosome 1 |
| Location: | 2193334 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g800041 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCATCAGACCCCTGTCCACCGAGGCACCATGTCCCTTCAGATGCCAGG |
| Internal bar code: | TCGCTAGTGTAGAACTCAGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2920 |
| LEAP-Seq percent confirming: | 96.5116 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCCTAAGCTCCAGATGAG |
| Suggested primer 2: | CCAAAGGGTTGCTTTGAGCC |