| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076462 |
| Chromosome: | chromosome 3 |
| Location: | 9196380 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199311 | VPS16,VCL1 | VPS16-like protein; (1 of 1) PTHR12811//PTHR12811:SF0 - VACUOLAR PROTEIN SORTING VPS16 // VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 16 HOMOLOG | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATCCATCGAAATCCTCTCGGCCCAGCCCACACCGCGCTCAACTCTCTG |
| Internal bar code: | GTAGTTAATGACAACGTAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4220 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGTAGGTCACTGCAGCA |
| Suggested primer 2: | ACCGTATACGCTACGCACAG |