| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.076466 |
| Chromosome: | chromosome 3 |
| Location: | 6425600 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192950 | (1 of 4) 2.7.11.13 - Protein kinase C / PKC | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTGTTTGCCCTCGAGACCGTCTTCAGCCAGTCAGCCAGTCAAGCGCTC |
| Internal bar code: | GGGCCTGATTGAGTGGCTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1580 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCGGCCGAGATGTCATTG |
| Suggested primer 2: | GAATCAGCGCCGGAGATGTA |