| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.076482 |
| Chromosome: | chromosome 12 |
| Location: | 9342527 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541850 | (1 of 1) 3.6.1.7 - Acylphosphatase / Acylphosphate phosphohydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGGCATCCGGAGACACCACTGAGCAGCGCAGTGGGGGCAGCGCGGCG |
| Internal bar code: | GGGGGCGGCAATACATGTTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 674 |
| LEAP-Seq percent confirming: | 88.2353 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTAGCCCCTAGTCCCAAGG |
| Suggested primer 2: | CGAGGTATTCGGCAGGGTAC |