| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.076490 |
| Chromosome: | chromosome 13 |
| Location: | 3936504 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g801566 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAATTCGGAGAGTTGGTAGGCTGATAGGTCCAGAACTTCGGTTCCTATG |
| Internal bar code: | TTCTGATTTCTGCGGGCCGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4817 |
| LEAP-Seq percent confirming: | 5.26316 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGTGTGGAGAGGGCATCG |
| Suggested primer 2: | TGTGTGTGACGCCAGCATAT |