| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.076491 |
| Chromosome: | chromosome 10 |
| Location: | 6429565 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g464750 | NAT25 | (1 of 5) PTHR23091:SF255 - ACYL-COA N-ACYLTRANSFERASES-LIKE PROTEIN; N-acetyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACGCCGTTGCCATCGCCTCCGGCGCCCCCAGCACCATCCGTGCTGTCC |
| Internal bar code: | TTGTTGGATCTAGAATGTCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 258 |
| LEAP-Seq percent confirming: | 4.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCATGCTGGAAACTAGGG |
| Suggested primer 2: | CGCCATGAATTCGCTTCCAG |