Insertion junction: CLIP2.076507_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.076507
Chromosome:chromosome 2
Location:1827146
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g087000 (1 of 12) IPR000104//IPR002893 - Antifreeze protein, type I // Zinc finger, MYND-type 3'UTR
Cre02.g087050 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATATAAATCATGGGCACTGATGCTGTTGCTGGCATCACATACGGTTTAA
Internal bar code:TGAAAAGAATAATTTAACGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:3676
LEAP-Seq percent confirming:98.0392
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTACTCGGGTTTGGCTGT
Suggested primer 2:AAAGTCAGGTGTGGGCAACA