| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.076509 |
| Chromosome: | chromosome 13 |
| Location: | 163197 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g563100 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGCTGGATTCTGTCTCACGGTCTCGCTAGCCGGCAGTGGCAACCCTTC |
| Internal bar code: | ATAGCCTACCCGCGGTACGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1816 |
| LEAP-Seq percent confirming: | 73.0769 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCTGGAAGTCACCGAATG |
| Suggested primer 2: | TGAAGACGTCCATGGAGCAC |