Insertion junction: CLIP2.076520_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.076520
Chromosome:chromosome 12
Location:7965656
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g552750 (1 of 2) PF04520 - Senescence regulator (Senescence_reg) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACTGCGGTTTGAAATGTTCTGTATTATCTCTTCTATTTACGGCGAATA
Internal bar code:TATGCGTTTACACTCTGCATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3909
LEAP-Seq percent confirming:2.48447
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:157
LEAP-Seq n unique pos:161

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTAGGTGCTTGAGAGGCA
Suggested primer 2:TAGCAGTCACAGGCCAACAG