| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.076532 |
| Chromosome: | chromosome 12 |
| Location: | 591909 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493700 | ZXE2 | Putative zeaxanthin epoxidase; (1 of 4) PF01494//PF13450 - FAD binding domain (FAD_binding_3) // NAD(P)-binding Rossmann-like domain (NAD_binding_8) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCAGGCTGCGGCGCGAGATGCCCAGACCGTTGGAGCGGACGTCCTTC |
| Internal bar code: | TAATTCAACCAGCCCGACAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 356 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTACCTGTACTACGCCGC |
| Suggested primer 2: | CCCGTCCTCAAACTCAAGCT |