| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076533 |
| Chromosome: | chromosome 16 |
| Location: | 7808005 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688302 | (1 of 1) K08999 - hypothetical protein (K08999) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATTAAATCAACTACCAAATGTCAGTACAACACGGCGACATTGGGCGGC |
| Internal bar code: | GCTCTTAATCACTCGGGCCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5386 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTTGACCTCGACGAAACC |
| Suggested primer 2: | CGTGTGTCTCACTAAGCGGT |