| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076547 |
| Chromosome: | chromosome 12 |
| Location: | 6173694 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535700 | HSP70D | (1 of 2) PTHR19375:SF173 - CHAPERONE PROTEIN DNAK; Heat shock protein 70D | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGAATTGCGTGGGGTCGACACCGCACAGATCTACACGTGCCCCGTGGT |
| Internal bar code: | TAAATCGTATATTCATGTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 46.6667 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACATGGACGGCTAACCTT |
| Suggested primer 2: | CCTGCTCGTTGTCCTTTCCT |