Insertion junction: CLIP2.076560_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.076560
Chromosome:chromosome 10
Location:4099107
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g448350 (1 of 3) PTHR10361 - SODIUM-BILE ACID COTRANSPORTER 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTAAAGAAGCAGCCGCACACGCAACCCCACAACCCTGCCGCCCCGCCT
Internal bar code:TAACCCGTCGAATCTAGTAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:195
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CATGCCTTATTGTTCCGCGG
Suggested primer 2:GCTGCCTGGTGTTTAGCCTA