Insertion junction: CLIP2.076566_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.076566
Chromosome:chromosome 7
Location:1412508
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g323250 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCGCTATGCGCATTTCAGACACAGACTTGCCCAATCATTTGTGGATTT
Internal bar code:CACCAGTTGTCACTCACAGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:2042
LEAP-Seq percent confirming:71.4286
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGCTATAGACGTGCTCGC
Suggested primer 2:CGGGCGCCATTATCAAAAGG