| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076568 |
| Chromosome: | chromosome 4 |
| Location: | 1252918 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215000 | BKT,CBK1,BKT1 | Beta-carotene ketolase; (1 of 1) IPR000104//IPR005804 - Antifreeze protein, type I // Fatty acid desaturase domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTCTCCCTGCACTCAGTGCAACACACTTCCCTGCAGCACCGCGGCCA |
| Internal bar code: | CATTAGGTTAGACGTGCGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 951 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTACCGTGTCTGTTCACA |
| Suggested primer 2: | CCGTTGGCAGCACATATTCG |