| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.076569 |
| Chromosome: | chromosome 6 |
| Location: | 6270082 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g292450 | PUS13 | RNA pseudouridine synthase; (1 of 1) 5.4.99.44 - Mitochondrial tRNA pseudouridine(27/28) synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTTCTGCGGCGGCGGCTGGCCCTCGCCCTCCCCTGCCACCGACTCAG |
| Internal bar code: | AGGTCCAGCCCCGTCAGATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1888 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCGTTTAAACTCCGGGAG |
| Suggested primer 2: | TTTTGTGAGCATGGCCGTTG |