| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.076575 |
| Chromosome: | chromosome 3 |
| Location: | 5518991 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g184950 | MAW3 | Membrane-associated hydroxyproline-rich glycoprotein 3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGGCTGTGGATGATAGGCGGCGGGCGTGGCGAAGGCGGGCTGAGCAG |
| Internal bar code: | GGTATAGATACCAGGATGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 579 |
| LEAP-Seq percent confirming: | 63.6364 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGAGGCTGTCTTCAACGT |
| Suggested primer 2: | CAACATCATTAGCGCCAGGC |