Insertion junction: CLIP2.076587_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.076587
Chromosome:chromosome 14
Location:2526602
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g624550 5'UTR
Cre14.g624600 (1 of 85) IPR009057 - Homeodomain-like 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGGGTAGCTCGCCCATCGCTACACTGGAAAATTCCGGGCTCGCGAAGG
Internal bar code:TTAAAGTGCTCGGGTAATAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1889
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCACAACAAACGAGGTGG
Suggested primer 2:CTTGCGACTTTGGAGCACAG