Insertion junction: CLIP2.076601_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.076601
Chromosome:chromosome 1
Location:2051321
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g011300 SCLP50,CPY Serine carboxypeptidase; (1 of 3) K09645 - vitellogenic carboxypeptidase-like protein [EC:3.4.16.-] (CPVL) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCATGCACGGACCCCCGGTGCCCACACGGCCACACGACGAGCCCAACA
Internal bar code:GATATCGAGGGCGCATGTTAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:674
LEAP-Seq percent confirming:63.6364
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGAGGGCGCAAAGTTTTC
Suggested primer 2:TGTCCAGCTATCCCGTCTCA