Insertion junction: CLIP2.076605_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.076605
Chromosome:chromosome 6
Location:6699485
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g295150 (1 of 4) PF13515 - Fusaric acid resistance protein-like (FUSC_2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCGGCAAGACGCCAGTCACGCCCCAGCACGCTTGATTGTTGCGTGACG
Internal bar code:AGCTATTTCTTGTTCGATCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2237
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:70
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:70

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGAGTGCAGAGAGACGCA
Suggested primer 2:CTCATGTCCTGGGTTCGCTT