Insertion junction: CLIP2.076619_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.076619
Chromosome:plastome
Location:189810
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802332 ChreCp067,2717046,psaC photosystem I iron-sulfur center; (1 of 1) K02691//K05575 - photosystem I subunit VII (psaC) // NAD(P)H-quinone oxidoreductase subunit 4 (ndhD) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGTATCGTAAATTTTAACGATATGAGCCATATTTAAATTTTAAGTTAA
Internal bar code:GCCACACCGATAATCCGACTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:67
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TATGCAAACATCCCGCATGG
Suggested primer 2:GCCTTTGGCTGGAAGAGTCT