| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076649 |
| Chromosome: | chromosome 3 |
| Location: | 2204474 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157300 | CAF1 | (1 of 1) K12581 - CCR4-NOT transcription complex subunit 7/8 (CNOT7_8, CAF1, POP2); CCR4-associated factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTGAGATGCCGGCACTGTCGTGACACCAAAACCACGTGAATCAAATGT |
| Internal bar code: | GTTGGATTACCAATTCTGCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4412 |
| LEAP-Seq percent confirming: | 96.7213 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGGGAGCGATCAGGATG |
| Suggested primer 2: | AGCTTCTCGGACAGGAGACT |