Insertion junction: CLIP2.076650_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.076650
Chromosome:chromosome 10
Location:6554803
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g465550 CLPD1,CLPD (1 of 2) 3.6.4.10 - Non-chaperonin molecular chaperone ATPase / Molecular chaperone Hsc70 ATPase; ClpD chaperone, Hsp100 family 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGAAACAATGAACACGCTAGTGCGGGCTGGGTCTATTTCCGACATCGGC
Internal bar code:AGTTAAGGGTTCAGAGAGCCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1657
LEAP-Seq percent confirming:95.6522
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTCTGTTCCCGCTTCTGA
Suggested primer 2:ATGAGACCCCGCTGTTCAAG