| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.076651 |
| Chromosome: | chromosome 10 |
| Location: | 2815576 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438850 | ROC76 | (1 of 10) PF00170 - bZIP transcription factor (bZIP_1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTGGGCGCTCTGGTGCTGCGTCGGGGCTCATGTCAGCACGCCGACGT |
| Internal bar code: | GTTTTCCGTCTAACTCTCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2227 |
| LEAP-Seq percent confirming: | 63.1579 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTGAGCCCTGTCAGGAAC |
| Suggested primer 2: | GCAAGCCCTTGTGCTGTTAC |