Insertion junction: CLIP2.076660_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.076660
Chromosome:chromosome 2
Location:3454614
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g098800 (1 of 1) K09586 - endoplasmic reticulum protein 29 (ERP29) 3'UTR
Cre02.g098850 NTR2 NADPH dependent thioredoxin reductase 2; (1 of 2) PTHR22912//PTHR22912:SF183 - DISULFIDE OXIDOREDUCTASE // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGCACCTGCACCTTCCTCTGCCCCATACTCCCATCGCGCCCTCACGCG
Internal bar code:TTAAAAGCTCTTCACGCAGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1911
LEAP-Seq percent confirming:70.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTGCTAACAATACGCGGC
Suggested primer 2:AAGCTACATCCCTTGGGTGC