| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.076700 |
| Chromosome: | chromosome 4 |
| Location: | 1154480 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215700 | (1 of 4) IPR009030//IPR011641 - Insulin-like growth factor binding protein, N-terminal // Tyrosine-protein kinase ephrin type A/B receptor-like | 3'UTR | |
| Cre04.g215750 | (1 of 1) K02180 - cell cycle arrest protein BUB3 (BUB3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAGCACGCAGGCTGCTGGTCCGCTCTTCGGCTCATGGACCCGAGTGC |
| Internal bar code: | TCCGACATATTGGATATAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4283 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 93 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGACTCTTTTTCCCTGCC |
| Suggested primer 2: | ATACCGTCCCGAGAACCAGA |