Insertion junction: CLIP2.076719_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.076719
Chromosome:chromosome 2
Location:7627339
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g147800 SEC14 (1 of 13) IPR001251//IPR011074 - CRAL-TRIO lipid binding domain // CRAL/TRIO, N-terminal domain; Putative phosphoglyceride transfer protein 5'UTR
Cre02.g800268 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTGGGCGGCTTGCATGCAGCCCAACCCTAGTTTGCCTGAGAGCCCGGA
Internal bar code:AGATTAGCCGTCATTAAAGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1157
LEAP-Seq percent confirming:86.7347
LEAP-Seq n confirming:85
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:98

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTAGGGGTGTGGGGAATA
Suggested primer 2:GGGACCACGTGTGTGTGTAT