| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.076735 |
| Chromosome: | chromosome 3 |
| Location: | 6578267 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194600 | (1 of 2) IPR000719//IPR011009//IPR013221 - Protein kinase domain // Protein kinase-like domain // Mur ligase, central | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCCCACAGCTCTGTTAACATGCACCTGCCGGCATTTGTGTGGGCGTGG |
| Internal bar code: | CTTCACAACTAACCCCATATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2590 |
| LEAP-Seq percent confirming: | 41.8182 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGTTAGCGAAGGCGAAG |
| Suggested primer 2: | AAGATGCGGACTGAAGGGTG |