Insertion junction: CLIP2.076745_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.076745
Chromosome:chromosome 1
Location:2636144
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g015750 (1 of 2) PF07978 - NIPSNAP (NIPSNAP) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGTGCCCCCGACCCTATCAATATGGCTCCCCAGCGCATTGCAACGACCG
Internal bar code:GGAAAATATAATGTCTTGGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:394
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTCACACAATTGCACGCCAA
Suggested primer 2:AAAGTGGACCGATGGCGATT