| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076746 |
| Chromosome: | chromosome 3 |
| Location: | 7333653 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206700 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCCGCTAGCACCCCGGCTAGCGCCGCCGCTTGCACCGGCGAAGCTGC |
| Internal bar code: | TACGCAGAGTAAGCTGGGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 373 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTTAGGTTTCGAGTCGC |
| Suggested primer 2: | GCCGCGTCATGATGAAAGTC |