| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.076765 |
| Chromosome: | chromosome 1 |
| Location: | 7575563 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g053150 | GPDH3,GPD3 | (1 of 3) K00006 - glycerol-3-phosphate dehydrogenase (NAD+) (GPD1); Glycerol-3-phosphate dehydrogenase/dihydroxyacetone-3-phosphate reductase | 3'UTR |
| Cre01.g053200 | (1 of 1) PTHR12415:SF0 - TYROSYL-DNA PHOSPHODIESTERASE 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCAGCGTAGTGTCACCGATGAGCAGGAGCAGTGGCAGGCCGTTTGAA |
| Internal bar code: | AAGACGCACCCGGGGCGGTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1318 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTCCACGTGTGTAATGT |
| Suggested primer 2: | GACCGCTGACAAACACACAC |