| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.076777 |
| Chromosome: | plastome |
| Location: | 148423 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802322 | icreI,341940450,I-CreI | (1 of 2) PF00961 - LAGLIDADG endonuclease (LAGLIDADG_1); DNA endonuclease I-CreI | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAATCGAGCAACAATAAATAAATTGTATGTAAACATTCTTTTAGAGATT |
| Internal bar code: | AGGGTCACGCCTCTGTTAACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 280 |
| LEAP-Seq percent confirming: | 4.7619 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGGTTTGTAGACGGTGA |
| Suggested primer 2: | TTCGGGATGGATTGGTGTGG |