| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.076778 |
| Chromosome: | chromosome 17 |
| Location: | 4171169 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g729200 | (1 of 1) PF04678 - Mitochondrial calcium uniporter (MCU) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCCGGGGTGTTGGTACCAGCCGAAGGCTTACGCTTTGGCATGCCCAT |
| Internal bar code: | GTTATCCGAGATATACCTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3240 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCGTCTGGTATGAGAGCG |
| Suggested primer 2: | GTGGGTTTTGGTGTGTACGC |