| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076781 |
| Chromosome: | chromosome 9 |
| Location: | 831199 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g403750 | HAD6 | Halo-acid dehalogenase-like hydrolase; (1 of 1) PTHR12725:SF55 - F14L17.7 PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACTGTAATTGTAGTGGGGTGCTCTGGAAACAATGGTACAGGGGAGGTG |
| Internal bar code: | GATCGATGAAAGCCGTGTTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1572 |
| LEAP-Seq percent confirming: | 11.1111 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCATGCGTCTCTCTTGAG |
| Suggested primer 2: | CGCTAGCGAGTTGAGGAGAG |