| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.076801 |
| Chromosome: | chromosome 11 |
| Location: | 2510773 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095104 | (1 of 1) K15193 - protein SPT2 (SPTY2D1, SPT2) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACCGCCGCGCATGACCCCGTGCCGCCTGCCGCGCAGCAGGCGCCAAGC |
| Internal bar code: | TGTAGCGGGTAGATTGGTTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGGGCTAGCTGCTTTTG |
| Suggested primer 2: | GACCACTTACACACGAGCCA |