| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.076806 |
| Chromosome: | chromosome 16 |
| Location: | 4406070 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668300 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACGTTCTTCTGTGAAACACTTCAAGTCATGAAATATCGCTCAAAGTCG |
| Internal bar code: | GTCAAGGCCCCGATGCTCTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3436 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTTGTTTCGGGACTTTT |
| Suggested primer 2: | TCCTTTGTGCAGGAATCCCC |