| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.076826 |
| Chromosome: | chromosome 12 |
| Location: | 4768375 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523200 | NURF55 | Nucleosome remodeling factor; (1 of 1) PTHR22850//PTHR22850:SF82 - WD40 REPEAT FAMILY // HISTONE-BINDING PROTEIN RBBP7 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGATATGCCGTGCGTCGCGGTAATGGTGTCCGTATGGATGGGAGGCAT |
| Internal bar code: | CTGGGTACTTGATGTCACTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4100 |
| LEAP-Seq percent confirming: | 73.3333 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACCTTGCAACAGCAGTG |
| Suggested primer 2: | ATCTGGCAGATGGCGTCAAA |