| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076829 |
| Chromosome: | chromosome 9 |
| Location: | 5031395 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g405800 | (1 of 1) PTHR11911//PTHR11911:SF109 - INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED // SUBFAMILY NOT NAMED | 5'UTR | |
| Cre09.g405850 | NUO7 | NADH:ubiquinone oxidoreductase 49 kDa ND7 subunit; (1 of 1) K03935 - NADH dehydrogenase (ubiquinone) Fe-S protein 2 (NDUFS2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAAACTTTGGAGAGAGTGTGGTTCTTAAGTTCGACGTGTAAGTAAGACT |
| Internal bar code: | ATCTTCGTTTTGTCTTTTGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 841 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTCCCTAAACACAGAGC |
| Suggested primer 2: | TCGCTTCTTTGCAAAGTGGC |