| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076838 |
| Chromosome: | chromosome 5 |
| Location: | 2861055 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241400 | (1 of 2) 1.16.1.7 - Ferric-chelate reductase (NADH) / NADH:Fe(3+)-EDTA reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGGCCGCAATGCGGTACCTTGAGAACCACCCGTTGCTTTTCGCACGA |
| Internal bar code: | ACTATCTAATTTATGTAGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 270 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGGGGCAGGATGTGTGA |
| Suggested primer 2: | CGACCTACTTGCTAGGCTCG |