| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076843 |
| Chromosome: | chromosome 6 |
| Location: | 1989933 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g264900 | HTB13 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTGAAGACAAGGTCCAGCCTCTGAGCGCGCTTAGCCCGAGGTGAACT |
| Internal bar code: | CGTTTAATGGGTAATCTCTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3897 |
| LEAP-Seq percent confirming: | 40.9091 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCATTGTGTTGGGTTGG |
| Suggested primer 2: | CATAGCACGTAGGAGCCCAG |