Insertion junction: CLIP2.076845_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.076845
Chromosome:chromosome 16
Location:4190561
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre16.g670200 Microtubule-associated protein cysteine-rich PDZ-binding protein; (1 of 2) PTHR11805:SF1 - CYSTEINE-RICH PDZ-BINDING PROTEIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTGTTCTCGTTGACCTTGCGCCCGCTGCTCTCGCCCTGCTTATCACCC
Internal bar code:TGGTGAGACATTGAGGTAAGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1052
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAAATAGTTCTGGGCCGGGT
Suggested primer 2:CGCTCATGCTAGCCATACCA