Insertion junction: CLIP2.076852_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.076852
Chromosome:chromosome 5
Location:878060
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre05.g246300 (1 of 1) PTHR13554//PTHR13554:SF10 - 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 5-RELATED // 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 5 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCTGAATGGCTCCCGCAGCTGAGTCTGGTGGTGAGGATCTAGCACAACT
Internal bar code:GAACGCTGTTACAATTGTGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:442
LEAP-Seq percent confirming:71.4286
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGCCAAATGCCATATGGG
Suggested primer 2:GATGAAAGCCGACGCAGAAC